Article

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN-m

  1. Tomonori Sakurai1,
  2. Akira Satake1,
  3. Shoichiro Sumi1,
  4. Kazutomo Inoue1,
  5. Junji Miyakoshi2,*

Article first published online: 19 MAR 2004

DOI: 10.1002/bem.10181

Issue

Bioelectromagnetics

Bioelectromagnetics

Volume 25, Issue 3, pages 160–166, April 2004

Additional Information

How to Cite

Sakurai, T., Satake, A., Sumi, S., Inoue, K. and Miyakoshi, J. (2004), An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN-m. Bioelectromagnetics, 25: 160–166. doi: 10.1002/bem.10181

Author Information

  1. 1

    Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

  2. 2

    Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki University, Hirosaki, Japan

*Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki, 036-8564, Japan.

Publication History

  1. Issue published online: 19 MAR 2004
  2. Article first published online: 19 MAR 2004
  3. Manuscript Revised: 4 AUG 2003
  4. Manuscript Received: 2 MAY 2003

Funded by

  • Research for the Future Program, Japan Society for the Promotion of Science
  • Ministry of Education (for Scientific Research S). Grant Number: 13854020

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Keywords:

  • 5 mT and 60 Hz;
  • insulinoma cell line;
  • KCl stimulation;
  • calcium channels;
  • synaptosomal associated protein;
  • synaptotagmin 1

Abstract

In this study, we investigated the effects of exposure to an extremely low frequency magnetic field (ELFMF) on hormone secretion from an islet derived insulinoma cell line, RIN-m. We stimulated RIN-m cells to secrete insulin under exposure to an ELFMF, using our established system for the exposure of cultured cells to an ELFMF at 5 mT and 60 Hz, or under sham exposure conditions for 1 h and observed the effects. In the presence of a depolarizing concentration of potassium (45 mM KCl), exposure to ELFMF significantly attenuated insulin release from RIN-m cells, compared to sham exposed cells. Treatment with nifedipine reduced the difference in insulin secretion between cells exposed to an ELFMF and sham exposed cells. The expression of mRNA encoding synaptosomal associated protein of 25 kDa (SNAP-25) and synaptotagmin 1, which play a role in exocytosis in hormone secretion and influx of calcium ions, decreased with exposure to an ELFMF in the presence of 45 mM KCl. These results suggest that exposure to ELFMF attenuates insulin secretion from RIN-m cells by affecting calcium influx through calcium channels. Bioelectromagnetics 25:160–166, 2004. © 2004 Wiley-Liss, Inc.

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