The biological impact of concurrent exposure to metallic nanoparticles and a static magnetic field
Article first published online: 2 MAY 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 34, Issue 7, pages 500–511, October 2013
How to Cite
Comfort, K. K., Maurer, E. I. and Hussain, S. M. (2013), The biological impact of concurrent exposure to metallic nanoparticles and a static magnetic field. Bioelectromagnetics, 34: 500–511. doi: 10.1002/bem.21790
- Issue published online: 17 SEP 2013
- Article first published online: 2 MAY 2013
- Manuscript Accepted: 3 MAR 2013
- Manuscript Received: 14 MAY 2012
- 711th HPW/AFRL Chief Scientist Seedling Program
- Tier I, Air Force Surgeon General
- National Research Council post-doctoral fellowship to K.K.C. through the Air Force Office of Scientific Research and Oak Ridge Institute for Science and Education
- Henry M. Jackson Foundation to E.I.M.
- static magnetic field;
- superparamagnetic iron oxide nanoparticle;
- gold nanoparticle;
- cellular stress;
- gene regulation
The rapid advancement of technology has led to an exponential increase of both nanomaterial and magnetic field utilization in applications spanning a variety of sectors. While extensive work has focused on the impact of these two variables on biological systems independently, the existence of any synergistic effects following concurrent exposure has yet to be investigated. This study sought to ascertain the induced alterations to the stress and proliferation responses of the human adult low calcium, high temperature keratinocyte (HaCaT) cell line by the application of a static magnetic field (approximately 0.5 or 30 mT) in conjunction with either gold or iron oxide nanoparticles for a duration of 24 h. By evaluating targets at a cellular, protein, and genetic level a complete assessment of the HaCaT response was generated. A magnetic field-dependent proliferative effect was found (∼15%), which correlated with a decrease in reactive oxygen species and a simultaneous increase in ki67 expression, all occurring independently of nanoparticle presence. Furthermore, the application of a static magnetic field was able to counteract the cellular stress response induced by nanoparticle exposure through a combination of decreased reactive oxygen species production and modification of gene regulation. Therefore, we conclude that while these variables each introduce the potential to uniquely influence physiological events, no negative synergistic reactions were identified. Bioelectromagnetics. 34:500–511. © 2013 Wiley Periodicals, Inc.