Jian Zhou and Jia-Qi Wang contributed equally to this work.
Different electromagnetic field waveforms have different effects on proliferation, differentiation and mineralization of osteoblasts in vitro
Article first published online: 17 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 35, Issue 1, pages 30–38, January 2014
How to Cite
Zhou, J., Wang, J.-Q., Ge, B.-F., Ma, X.-N., Ma, H.-P., Xian, C. J. and Chen, K.-M. (2014), Different electromagnetic field waveforms have different effects on proliferation, differentiation and mineralization of osteoblasts in vitro. Bioelectromagnetics, 35: 30–38. doi: 10.1002/bem.21794
- Issue published online: 2 DEC 2013
- Article first published online: 17 JUN 2013
- Manuscript Accepted: 4 APR 2013
- Manuscript Received: 16 JUN 2012
- Gansu Research Foundation of Science and Technology
- electromagnetic fields;
Noninvasive electromagnetic fields (EMFs) have been known to be able to improve bone health; however, their optimal application parameters and action mechanisms remain unclear. This study compared the effects of different forms of EMFs (sinusoidal, triangular, square, and serrated, all set at 50 Hz frequency and 1.8 mT intensity) on proliferation, differentiation and mineralization of rat calvarial osteoblasts. Square EMFs stimulated osteoblast proliferation but sinusoidal EMFs inhibited it. Sinusoidal and triangular EMFs produced significantly greater alkaline phosphatase (ALP) activity, ALP staining areas, calcium deposition, mineralized nodule areas, and mRNA expression of Runx-2, osteoprotegerin and insulin-like growth factor-I than square and serrated EMFs (P < 0.01). Triangular EMFs had a greater effect than sinusoidal EMFs on every indices except for Runx-2 mRNA expression (P < 0.05). These results indicated that while square EMFs promoted proliferation and had no effect on the differentiation of osteoblasts, sinusoidal EMFs inhibited proliferation but enhanced osteogenic differentiation. Triangular EMFs did not affect cell proliferation but induced the strongest osteogenic activity among the four waveforms of EMFs. Thus, the effects of EMFs on proliferation and differentiation of osteoblasts in vitro were dependent on their waveforms. Bioelectromagnetics. 35:30–38, 2014. © 2013 Wiley Periodicals, Inc.