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Interspecific resources: a major tool for quantitative trait locus cloning and speciation research

Authors

  • David L'Hôte,

    1. INSERM, U567, Institut Cochin, Paris, France
    2. CNRS, UMR8104, Institut Cochin, Paris, France
    3. Université Paris Descartes, Faculté de Médecine Hôpital Cochin, Paris, France
    4. Institut Jacques Monod, UMR 7592 CNRS Université Paris Diderot, Paris, France
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    • These two authors contributed equally to this manuscript.

  • Paul Laissue,

    1. INSERM, U567, Institut Cochin, Paris, France
    2. CNRS, UMR8104, Institut Cochin, Paris, France
    3. Université Paris Descartes, Faculté de Médecine Hôpital Cochin, Paris, France
    4. Facultad de Medicina, Departamento de Ciencias Básicas, Unidad de Genética, Universidad del Rosario, Bogotá, Colombia
    5. Genética Molecular de Colombia, Bogotá, Colombia
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    • These two authors contributed equally to this manuscript.

  • Catherine Serres,

    1. INSERM, U567, Institut Cochin, Paris, France
    2. CNRS, UMR8104, Institut Cochin, Paris, France
    3. Université Paris Descartes, Faculté de Médecine Hôpital Cochin, Paris, France
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  • Xavier Montagutelli,

    1. Institut Pasteur, Unité de Génétique Fonctionnelle de la Souris, CNRS URA 2578, Paris, France
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  • Reiner A. Veitia,

    1. Institut Jacques Monod, UMR 7592 CNRS Université Paris Diderot, Paris, France
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  • Daniel Vaiman

    Corresponding author
    1. INSERM, U567, Institut Cochin, Paris, France
    2. CNRS, UMR8104, Institut Cochin, Paris, France
    3. Université Paris Descartes, Faculté de Médecine Hôpital Cochin, Paris, France
    • Cochin Institute, Genetics and Development, 24 rue du Fg St Jacques, 75014 Paris, France.
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Abstract

Positional cloning of the quantitative trait locus (QTL) still encounters numerous difficulties, which explains why thousands of QTL have been mapped, while only a few have been identified at the molecular level. Here, we focus on a specific mapping tool that exists in plant and animal model species: interspecific recombinant congenic strains (IRCSs) or interspecific nearly isogenic lines (NILs). Such panels exhibit a much higher sequence diversity than intraspecific sets, thus enhancing the contrasts between phenotypes. In animals, it allows statistical significance to be reached even when using a limited number of individuals. Therefore, we argue that interspecific resources may constitute a major genetic tool for positional cloning and for understanding some bases of speciation mechanisms.

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