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Super-resolution imaging prompts re-thinking of cell biology mechanisms

Selected cases using stimulated emission depletion microscopy

Authors

  • Sinem Saka,

    1. European Neuroscience Institute, DFG Center for Molecular Physiology of the Brain/Excellence Cluster, Göttingen, Germany
    2. International Max Planck Research School Molecular Biology, Göttingen, Germany
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  • Silvio O. Rizzoli

    Corresponding author
    1. European Neuroscience Institute, DFG Center for Molecular Physiology of the Brain/Excellence Cluster, Göttingen, Germany
    • European Neuroscience Institute, DFG Center for Molecular Physiology of the Brain/Excellence Cluster 171, Grisebachstr. 5, 37077 Göttingen, Germany.
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Abstract

The use of super-resolution imaging techniques in cell biology has yielded a wealth of information regarding cellular elements and processes that were invisible to conventional imaging. Focusing on images obtained by stimulated emission depletion (STED) microscopy, we discuss how the new high-resolution data influence the ways in which we use and interpret images in cell biology. Super-resolution images have lent support to some of our current hypotheses. But, more significantly, they have revealed unexpectedly complex processes that cannot be accounted for by the simpler models based on diffraction-limited imaging. The super-resolution imaging data challenge cell biologists to change their theoretical framework, by including, for instance, interpretations that describe multiple functions, functional errors or lack of function for cellular elements. In this context, we argue that descriptive research using super-resolution microscopy is now as necessary as hypothesis-driven research.

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