These two authors contributed equally to this work.
Prospects & Overviews
Microtubule dynamic instability: A new model with coupled GTP hydrolysis and multistep catastrophe
Article first published online: 27 MAR 2013
Copyright © 2013 WILEY Periodicals, Inc.
Volume 35, Issue 5, pages 452–461, May 2013
How to Cite
Bowne-Anderson, H., Zanic, M., Kauer, M. and Howard, J. (2013), Microtubule dynamic instability: A new model with coupled GTP hydrolysis and multistep catastrophe. Bioessays, 35: 452–461. doi: 10.1002/bies.201200131
- Issue published online: 12 APR 2013
- Article first published online: 27 MAR 2013
- Max Planck Society
Vol. 35, Issue 6, 579, Article first published online: 3 MAY 2013
- GTP hydrolysis;
A key question in understanding microtubule dynamics is how GTP hydrolysis leads to catastrophe, the switch from slow growth to rapid shrinkage. We first provide a review of the experimental and modeling literature, and then present a new model of microtubule dynamics. We demonstrate that vectorial, random, and coupled hydrolysis mechanisms are not consistent with the dependence of catastrophe on tubulin concentration and show that, although single-protofilament models can explain many features of dynamics, they do not describe catastrophe as a multistep process. Finally, we present a new combined (coupled plus random hydrolysis) multiple-protofilament model that is a simple, analytically solvable generalization of a single-protofilament model. This model accounts for the observed lifetimes of growing microtubules, the delay to catastrophe following dilution and describes catastrophe as a multistep process.