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Light resonance energy transfer-based methods in the study of G protein-coupled receptor oligomerization

  1. Jorge Gandía1,
  2. Carme Lluís2,
  3. Sergi Ferré3,
  4. Rafael Franco2,
  5. Francisco Ciruela1,*

Article first published online: 14 DEC 2007

DOI: 10.1002/bies.20682

Issue

BioEssays

BioEssays

Volume 30, Issue 1, pages 82–89, January 2008

Additional Information

How to Cite

Gandía, J., Lluís, C., Ferré, S., Franco, R. and Ciruela, F. (2008), Light resonance energy transfer-based methods in the study of G protein-coupled receptor oligomerization. Bioessays, 30: 82–89. doi: 10.1002/bies.20682

Author Information

  1. 1

    Unitat de Farmacologia, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Universitat de Barcelona, Spain

  2. 2

    Institut d'Investigacions Biomèdiques August Pi i Sunyer and Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain

  3. 3

    Behavioral Neuroscience Branch, National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Department of Health and Human Services, Baltimore, MD

*Unitat de Farmacologia, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina (Campus de Bellvitge), Pavelló de Govern, Av. Feixa Llarga, s/n, L'Hospitalet del Llobregat, 08907 Barcelona, Spain.

Publication History

  1. Issue published online: 14 DEC 2007
  2. Article first published online: 14 DEC 2007

Funded by

  • This work was supported by grant SAF2006-05481 to R. F. and SAF2005-00903 to FC from Ministerio de Educación y Ciencia and by the NIDA IRP funds

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Abstract

Since most of the functions in cells are mediated by multimeric protein complexes, the determination of protein–protein interactions is an important step in the study of cellular mechanisms. Traditionally, after screening for possible target interactors by means of a yeast two-hybrid screen, several methods are used to validate the initial result before carrying out functional experiments. Nowadays, non-invasive fluorescence-based methods like Bioluminescence Resonance Energy Transfer (BRET) and Fluorescence Resonance Energy Transfer (FRET) are widely used in the study of protein–protein interactions in living cells. In the present review, we address the individual strengths and weaknesses of both RET approaches, providing information on their possible future use in the study of G protein-coupled receptor oligomerization. BioEssays 30:82–89, 2008. © 2007 Wiley Periodicals, Inc.

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