Light Microscopy: From imaging probes to high resolution techniques andcomputational image analysis. On pages 333–340 Jason Swedlow gives a nice overview over the topics covered in this Special Issue. Advances in optics, automation, computational tools and imaging probes allow the visualization of ever smaller structures at increasing resolution. Furthermore, these techniques can be used for a wide variety of applications from basic research to clinical domains, e.g. by employing imaging techniques for screening.
The image shows a section of mouse small intestine stained with DAPI (blue), anti-tubulin (red) and phalloidin (green).