Authors Yeo Cho Yoon and Jin-Teak Hwang contributed equally to this work.
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Research Communication
Inhibitory effect of luteolin on the odorant-induced cAMP level in HEK293 cells expressing the olfactory receptor
Article first published online: 17 MAY 2012
DOI: 10.1002/biof.1025
Copyright © 2012 International Union of Biochemistry and Molecular Biology, Inc.
Additional Information
How to Cite
Yoon, Y. C., Hwang, J.-T., Sung, M.-J., Wang, S., Munkhtugs, D., Rhyu, M.-R. and Park, J.-H. (2012), Inhibitory effect of luteolin on the odorant-induced cAMP level in HEK293 cells expressing the olfactory receptor. BioFactors, 38: 360–364. doi: 10.1002/biof.1025
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Authors Yeo Cho Yoon and Jin-Teak Hwang contributed equally to this work.
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Publication History
- Issue published online: 17 OCT 2012
- Article first published online: 17 MAY 2012
- Manuscript Accepted: 12 APR 2012
- Manuscript Received: 30 JAN 2012
- Abstract
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Keywords:
- luteolin;
- cAMP;
- adenylyl cyclase;
- eugenol;
- flavonoid
Abstract
Luteolin is a flavonoid in many fruits and vegetables. Although luteolin has important biological functions, including antioxidant, anti-inflammatory, antimicrobial, and neuroprotective activities, little is known about the functions of luteolin in the olfactory system. Various odorants can be detected and distinguished by using several molecular processes, including the binding of odorants to odorant receptors, activation of adenylyl cyclase (AC), changes of cyclic adenosine monophosphate (cAMP) and Ca2+ levels in olfactory sensory neurons, as well as changes in membrane potentials and the transmission of electric signals to the brain. Because AC-cAMP signal transduction plays a pivotal role in the olfactory system, we evaluated the effects of luteolin on the AC-cAMP pathway that had been stimulated by the odorant eugenol. We demonstrated that eugenol caused an upregulation of the cAMP level and the phosphorylation of phosphokinase A (PKA, a downstream target of cAMP) in human embryonic kidney 293 (HEK293) cells expressing the murine eugenol receptor. This upregulation significantly decreased in the presence of luteolin, suggesting that luteolin inhibited the odorant-induced production of cAMP and affected the downstream phosphorylation of PKA.