Differential expression of centrosome-associated proteins in human brain tumors: A possible role of hNinein isoform 6 in cell differentiation

Authors

  • Joon-Khim Loh,

    1. Department of Neurosurgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Graduate Institute of Medicine, College of Medicine, Kaohsiung, Medical University, Kaohsiung, Taiwan
    3. Faculty of Healthcare Administration and Medical Informatics, College of Health Science, Kaohsiung Medical University, Kaohsiung, Taiwan
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  • Ann-Shung Lieu,

    1. Department of Neurosurgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Graduate Institute of Medicine, College of Medicine, Kaohsiung, Medical University, Kaohsiung, Taiwan
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  • Chia-Hua Chou,

    1. Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
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  • Ching-Chih Lin,

    1. Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Ming-Chang Yang,

    1. Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
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  • Fang-Yi Lin,

    1. Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Yi-Ren Hong,

    Corresponding author
    1. Graduate Institute of Medicine, College of Medicine, Kaohsiung, Medical University, Kaohsiung, Taiwan
    2. Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    3. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
    • Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan
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    • Tel: 886-7-3121101 Ext. 5386, Fax: 886-7-3218309

  • Shen-Long Howng

    Corresponding author
    1. Department of Neurosurgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Graduate Institute of Medicine, College of Medicine, Kaohsiung, Medical University, Kaohsiung, Taiwan
    • Department of Neurosurgery, Kaohsiung Medical University Hospital, No100 Tzyou 1st Road, Kaohsiung 807, Taiwan
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    • Tel: 886-7-3215049, Fax: 886-7-3215039


Abstract

Dysregulated centrosomal expression has been observed in high grade gliomas. Thus, this study aimed to examine the expression of Aurora family kinase and various centrosomal proteins, including centrin, γ-tubulin, and hNinein isoforms, in human brain tumors, including 29 meningiomas, 34 astrocytomas, 6 pituitary adenomas, and 6 metastatic tumors. mRNA expression was evaluated using reverse transcription polymerase chain reaction. The role of hNinein isoform 6 expression in cell differentiation was assessed in BrdU-treated IMR-32 cells. Differential expression of centrosomal proteins of brain tumors and cell lines was observed. Specifically, centrin 2 and centrin 3 expression levels were classified as moderate or abundant in >97% of samples in the meningioma group, 63% of astrocytomas, >83% of metastatic and pituitary tumors. Alternatively, hNinein isoform 6 expression was only detected in normal brain and astrocytoma tumors (17/34); however, it was not expressed in meningioma (0/29), metastatic tumors (0/6) (P < 0.001). Of the six neuroblastoma cell lines analyzed only IMR-32 cells expressed hNinein isoform 6. Furthermore, downregulated expression of hNinein isoform 6 and upregulation of γ-tubulin was correlated to astrocytoma tumor grade (P < 0.001). Increased hNinein isoform 6 mRNA expression was observed in response to BrdU treatment, and its expression was greater in teratomas as compared to embryonic stem cells. Further studies are necessary to determine if hNinein isoform 6 functions as a tumor-suppressor gene in brain tumors. Differential centrosomal protein expression may result in altered centrosome function that is observed the in progression of various brain tumors. © 2012 International Union of Biochemistry and Molecular Biology, Inc.

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