Selenium: A key element that controls NF-κB activation and IκBα half life
Article first published online: 16 DEC 2008
Copyright © 2001 International Union of Biochemistry and Molecular Biology, Inc.
Volume 14, Issue 1-4, pages 117–125, 2001
How to Cite
Kretz-Remy, C. and Arrigo, A.-P. (2001), Selenium: A key element that controls NF-κB activation and IκBα half life. BioFactors, 14: 117–125. doi: 10.1002/biof.5520140116
- Issue published online: 16 DEC 2008
- Article first published online: 16 DEC 2008
- Manuscript Accepted: 26 JAN 2001
- Association pour la Recherche sur le Cancer and the région Rhône-Alpes
- glutathione peroxidase;
- redox regulation;
Treatment of mammalian cells with hydrogen peroxide induces the nuclear translocation of the transcription factor NF-κ and its binding to κ DNA sequences present in the promoter region of numerous genes. The role of selenium in NF-κ activation was analyzed in human T47D cells overexpressing the seleno-dependent detoxifiant enzyme glutathione peroxidase. Following exposure to H2O2, these cells showed a seleno-dependent decreased accumulation of intracellular ROS and NF-κ activation. This phenomenon was correlated with an inhibition of the nuclear translocation of NF-κ (p50 subunit) and with an absence of Iκα degradation. We also report that the half-life of Iκα in untreated cells was increased two-fold by the overexpression of active glutathione peroxidase. These results suggest that selenium is a key element that through its modulation of glutathione peroxidase activity can inhibit NF-κ activation and can up-regulate Iκα normal half life.