Resveratrol protects against 4-HNE induced oxidative stress and apoptosis in Swiss 3T3 fibroblasts
Article first published online: 16 DEC 2008
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
Volume 20, Issue 1, pages 1–10, 2004
How to Cite
Kutuk, O., Adli, M., Poli, G. and Basaga, H. (2004), Resveratrol protects against 4-HNE induced oxidative stress and apoptosis in Swiss 3T3 fibroblasts. BioFactors, 20: 1–10. doi: 10.1002/biof.5520200101
- Issue published online: 16 DEC 2008
- Article first published online: 16 DEC 2008
- Manuscript Accepted: 21 JUL 2003
- Manuscript Revised: 17 JUL 2003
- Manuscript Received: 26 MAY 2003
- Sabanci University
- lipid peroxidation;
- cell death;
- DNA fragmentation
Here we report on the marked protective effect of resveratrol on 4-hydroxynonenal (4-HNE) induced oxidative stress and apoptotic death in Swiss 3T3 fibroblasts. 4-HNE, one of the major aldehydic products of the peroxidation of membrane w-6 polyunsaturated fatty acids, has been suggested to contribute to oxidant stress mediated cell injury. Indeed, in vitro treatment of 3T3 fibroblasts with 4-HNE induced a condition of oxidative stress as monitored by the oxidation of dichlorofluorescein diacetate; this reaction was prevented when cells were pretreated with resveratrol. Further, 4-HNE-treated fibroblasts eventually underwent apoptotic death as determined by differential staining and internucleosomal DNA fragmentation. Resveratrol pretreatment also prevented 4-HNE induced DNA fragmentation and apoptosis. These observations are consistent with a potential role of lipid peroxidation-derived products in programmed cell death and demonstrate that resveratrol can counteract this effect by quenching cell oxidative stress.