S-allyl cysteine attenuated CCl4-induced oxidative stress and pulmonary fibrosis in rats
Article first published online: 19 DEC 2008
Copyright © 2006 International Union of Biochemistry and Molecular Biology, Inc.
Volume 26, Issue 1, pages 81–92, 2006
How to Cite
Mizuguchi, S., Takemura, S., Minamiyama, Y., Kodai, S., Tsukioka, T., Inoue, K., Okada, S. and Suehiro, S. (2006), S-allyl cysteine attenuated CCl4-induced oxidative stress and pulmonary fibrosis in rats. BioFactors, 26: 81–92. doi: 10.1002/biof.5520260108
- Issue published online: 19 DEC 2008
- Article first published online: 19 DEC 2008
- Osaka City University Medical School
- Okayama University
- Pulmonary fibrosis;
- S-allyl cysteine;
- carbon tetrachloride;
- chronic inflammation;
This study examined effects of S-allyl cysteine (SAC) on carbon tetrachloride (CCl4)-induced interstitial pulmonary fibrosis in Wistar rats. CCl4 (0.5 ml/kg) was intraperitoneally injected into rats twice a week for 8 weeks, and SAC (50, 100, or 200 mg/kg), N-acetyl cysteine (NAC, 200 or 600 mg/kg), or L-cysteine (CYS, 600 mg/kg) were orally administrated to rats everyday for 8 weeks. SAC significantly reduced the increases of transforming growth factor beta, lipid peroxides, AST, and ALT in plasma, induced by CCl4. Although CCl4 is mainly metabolized by hepatic cytochrome P450, CCl4 induced systemic inflammation and some organ fibrosis. SAC dose-dependently and significantly attenuated CCl4-induced systemic inflammation and fibrosis of lung. SAC also inhibited the decrease of thiol levels, the increase of inducible nitric oxide synthase expression, the infiltration of leukocytes, and the generation of reactive oxygen species in lungs. Although NAC and CYS attenuated CCl4-induced pulmonary inflammation and fibrosis, the order of preventive potency was SAC > NAC > CYS according to their applied doses. These results indicate that SAC is more effective than other cysteine compounds in reducing CCl4-induced lung injury, and might be useful in prevention of interstitial pulmonary fibrosis.