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Electric field driven jetting: an emerging approach for processing living cells

  1. Suwan N. Jayasinghe Dr.1,*,
  2. Peter A.M. Eagles2,
  3. Amer N. Qureshi2

Article first published online: 27 DEC 2005

DOI: 10.1002/biot.200500025

Issue

Biotechnology Journal

Biotechnology Journal

Volume 1, Issue 1, pages 86–94, January 2006

Additional Information

How to Cite

Jayasinghe, S. N., Eagles, P. A.M. and Qureshi, A. N. (2006), Electric field driven jetting: an emerging approach for processing living cells. Biotechnology Journal, 1: 86–94. doi: 10.1002/biot.200500025

Author Information

  1. 1

    Materials Department, Queen Mary, University of London, United Kingdom

  2. 2

    Randall Division of Cell and Molecular Biophysics, Kings College London, United Kingdom

*Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom, Fax: +44 207388 0180

Publication History

  1. Issue published online: 27 DEC 2005
  2. Article first published online: 27 DEC 2005
  3. Manuscript Accepted: 25 NOV 2005
  4. Manuscript Revised: 24 NOV 2005
  5. Manuscript Received: 11 OCT 2005

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Keywords:

  • Advanced electrified bio-jets;
  • Bioengineering;
  • Bioprocessing;
  • Living human jurkat cells;
  • Biofabrication

Abstract

This paper reports for the first time the ability to process living cellular materials by means of electrified jets at electric field strengths of up to 2 kV/mm. Bio-suspensions containing living human Jurkat cells at different concentrations were processed via this jetting approach. The jetting process was carried out at an electric field strength between 0.67 kV/mm and 2 kV/mm, corresponding to an applied voltage of 10–30 kV between two electrodes ∼15 mm apart. The Jurkat cells were jetted under sterile conditions, collected in petri dishes and incubated for 24 and 48 hours. During and after incubation, cells were assessed for survival and structural damage; cells were found to be unharmed and to retain their integrity under all electric field strengths examined. At all field strengths jetting took place in the unstable mode. Good correlation was observed between droplet distribution plots generated by way of laser spectroscopy and estimated values from measurements of droplet relics.

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