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Poly(3-hydroxybutyrate) production by Bacillus cereus SPV using sugarcane molasses as the main carbon source

Authors

  • Everest Akaraonye,

    1. Applied Biotechnology Research Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK
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  • Catalina Moreno,

    1. Applied Biotechnology Research Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK
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  • Jonathan C. Knowles,

    1. Department of Biomaterial and Tissue Engineering, Eastman Dental Institute, University College London, London, UK
    2. WCU Research Centre of Nanobiomedical Science, Dankook University, Dongnam-gu, Cheonan-si, Chungnam, Republic of Korea
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  • Tajalli Keshavarz,

    1. Applied Biotechnology Research Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK
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  • Dr. Ipsita Roy

    Corresponding author
    1. Applied Biotechnology Research Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK
    • School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK
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Abstract

The main hindrance in the use of polyhydroxyalkanoates (PHAs) as a replacement for existing petroleum-based plastics is their high production cost. The carbon source accounts for 50% of the cost for PHA production. Thus, increasing the yield and productivity of PHAs on cheap substrates is an important challenge for biotechnologists to support the commercialization and further applications of these polymers. In this study, we have investigated the use of an agricultural raw material, sugarcane molasses, as the main carbon source for poly(3-hydroxybutyrate) (P(3HB)) production by Bacillus cereus SPV. These studies were carried out in both shaken flasks and 2 L bioreactors. Various conditions were evaluated for their effects on biomass and P(3HB) accumulation. A high polymer yield was obtained, 61.07% dry cell weight (DCW) in a 1 L shaken flask study and 51.37% DCW in a 2 L fermenter study. These yields are 50% higher than previously observed with Bacillus cereus SPV. Hence, the results are encouraging and show that sugarcane molasses are a promising carbon source for an economical and commercially viable production of P(3HB).

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