Integrated biofabrication for electro-addressed in-film bioprocessing

Authors

  • Jessica L. Terrell,

    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Tanya Gordonov,

    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Yi Cheng,

    1. Institute for Systems Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Hsuan-Chen Wu,

    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Darryl Sampey,

    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. BioFactura, Inc., Key West Ave, Rockville, MD, USA
    Search for more papers by this author
  • Xiaolong Luo,

    1. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Chen-Yu Tsao,

    1. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Reza Ghodssi,

    1. Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Gary W. Rubloff,

    1. Institute for Systems Research, University of Maryland, College Park, MD, USA
    2. Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Gregory F. Payne,

    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    Search for more papers by this author
  • Dr. William E. Bentley

    Corresponding author
    1. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA
    2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
    • Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
    Search for more papers by this author

Abstract

Many recent advances in bioprocessing have been enabled by developments in miniaturization and microfluidics. A continuing challenge, however, is integrating multiple unit operations that require distinct spatial boundaries, especially with included labile biological components. We have suggested “biofabrication” as a means for organizing cells and biomolecules in complex configurations while preserving function of individual components. Polysaccharide films of chitosan and alginate that are assembled on-chip by electrodeposition are “smart” configurable interfaces that mediate communication between the biological systems and microfabricated devices. Here, we demonstrate the scalable performance of a production address, where incubated cells secrete antibodies, and a capture address, where secreted antibody is retained with specificity and subsequently assayed. The antibody exchange from one electro-address to another exemplifies integrated in-film bioprocessing, facilitated by the integrated biofabrication techniques used. This in-film approach enables complex processes without need for microfluidics and valving. Finally, we have shown scalability by reducing electrode sizes to a 1 mm scale without compromising film biofabrication or bioprocessing performance. The in situ reversible deposition of viable cells, productivity characterization, and capture of secreted antibodies could find use in bioprocessing applications such as clonal selection, run-to-run monitoring, initial scale-up, and areas including drug screening and biopsy analysis.

Ancillary