Addressable self-immobilization of lactate dehydrogenase across multiple length scales

Authors

  • Sibel Cetinel,

    1. Materials Science and Engineering, University of Washington, Seattle, WA, USA
    2. Genetically Engineered Materials Science and Engineering Center, University of Washington, Seattle, WA, USA
    3. Molecular Biology and Biotechnology Research Center (MOBGAM), Istanbul Technical University, Maslak, Istanbul, Turkey
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  • H. Burak Caliskan,

    1. Molecular Biology and Biotechnology Research Center (MOBGAM), Istanbul Technical University, Maslak, Istanbul, Turkey
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  • Deniz T. Yucesoy,

    1. Materials Science and Engineering, University of Washington, Seattle, WA, USA
    2. Genetically Engineered Materials Science and Engineering Center, University of Washington, Seattle, WA, USA
    3. Molecular Biology and Biotechnology Research Center (MOBGAM), Istanbul Technical University, Maslak, Istanbul, Turkey
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  • A. Senem Donatan,

    1. Materials Science and Engineering, Istanbul Technical University, Maslak, Istanbul, Turkey
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  • Esra Yuca,

    1. Bioengineering, Yildiz Technical University, Esenler, Istanbul, Turkey
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  • Mustafa Urgen,

    1. Materials Science and Engineering, Istanbul Technical University, Maslak, Istanbul, Turkey
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  • Nevin G. Karaguler,

    1. Molecular Biology and Biotechnology Research Center (MOBGAM), Istanbul Technical University, Maslak, Istanbul, Turkey
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  • Prof. Candan Tamerler

    Corresponding author
    1. Materials Science and Engineering, University of Washington, Seattle, WA, USA
    2. Genetically Engineered Materials Science and Engineering Center, University of Washington, Seattle, WA, USA
    • Materials Science and Engineering, Roberts Hall, Box 352120, University of Washington, Seattle, WA 98195, USA
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Abstract

Successful nanobiotechnology implementation largely depends on control over the interfaces between inorganic materials and biological molecules. Controlling the orientations of biomolecules and their spatial arrangements on the surface may transform many technologies including sensors, to energy. Here, we demonstrate the self-organization of L-lactate dehydrogenase (LDH), which exhibits enhanced enzymatic activity and stability on a variety of gold surfaces ranging from nanoparticles to electrodes, by incorporating a gold-binding peptide tag (AuBP2) as the fusion partner for Bacillus stearothermophilus LDH (bsLDH). Binding kinetics and enzymatic assays verified orientation control of the enzyme on the gold surface through the genetically incorporated peptide tag. Finally, redox catalysis efficiency of the immobilized enzyme was detected using cyclic voltammetry analysis in enzyme-based biosensors for lactate detection as well as in biofuel cell energy systems as the anodic counterpart. Our results demonstrate that the LDH enzyme can be self-immobilized onto different gold substrates using the short peptide tag under a biologically friendly environment. Depending on the desired inorganic surface, the proposed peptide-mediated path could be extended to any surface to achieve single-step oriented enzyme immobilization for a wide range of applications.

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