Arrayed cellular environments for stem cells and regenerative medicine

Authors

  • Drew M. Titmarsh,

    1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
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  • Huaying Chen,

    1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
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  • Ernst J. Wolvetang,

    1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
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  • Prof. Justin J. Cooper-White

    Corresponding author
    1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
    2. School of Chemical Engineering, The University of Queensland, St. Lucia, Australia
    • Australian Institute for Bioengineering and Nanotechnology, Building 75, Corner Cooper and College Roads, The University of Queensland, St. Lucia QLD 4072, Australia
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Abstract

The behavior and composition of both multipotent and pluripotent stem cell populations are exquisitely controlled by a complex, spatiotemporally variable interplay of physico-chemical, extracellular matrix, cell-cell interaction, and soluble factor cues that collectively define the stem cell niche. The push for stem cell-based regenerative medicine models and therapies has fuelled demands for increasingly accurate cellular environmental control and enhanced experimental throughput, driving an evolution of cell culture platforms away from conventional culture formats toward integrated systems. Arrayed cellular environments typically provide a set of discrete experimental elements with variation of one or several classes of stimuli across elements of the array. These are based on high-content/high-throughput detection, small sample volumes, and multiplexing of environments to increase experimental parameter space, and can be used to address a range of biological processes at the cell population, single-cell, or subcellular level. Arrayed cellular environments have the capability to provide an unprecedented understanding of the molecular and cellular events that underlie expansion and specification of stem cell and therapeutic cell populations, and thus generate successful regenerative medicine outcomes. This review focuses on recent key developments of arrayed cellular environments and their contribution and potential in stem cells and regenerative medicine.

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