A screening approach reveals the influence of mineral coating morphology on human mesenchymal stem cell differentiation

Authors

  • Siyoung Choi,

    1. Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA
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  • Prof. William L. Murphy

    Corresponding author
    1. Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA
    2. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA
    3. Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, USA
    4. AO Foundation Collaborative Research Center, Davos, Switzerland
    • Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Madison, WI 53706, USA
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Abstract

“Biomimetic” inorganic coating on biomaterials has been an active area of research with the aim of providing bioactive surfaces that can regulate cell behavior. Previous studies have demonstrated that human mesenchymal stem cell (hMSC) behavior is differentially regulated by the physical and chemical properties of inorganic mineral coatings, indicating that modulation of mineral properties is potentially important in regulating hMSC behavior. However, the lack of an efficient experimental context, in which to study stem cell behavior on inorganic substrates, has made it difficult to systematically study the effects of specific mineral coating parameters on hMSC behavior. In this study, we developed an efficient experimental platform to screen for the effects of mineral coating morphology on hMSC expansion and differentiation. hMSC expansion on mineral coatings was regulated by the micro-scale morphology of these coatings, with greater expansion on small granule-like coatings when compared to plate-like or net-like coatings. In contrast, hMSC osteogenic differentiation was inversely correlated with cell expansion on mineral coatings indicating that mineral coating morphology was a key parameter that regulates hMSC differentiation. The effect of mineral coating morphology on hMSC behavior underlines the utility of this inorganic screening platform to identify optimal coatings for medical devices and bone tissue engineering applications.

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