Product information: 3D organomimetic models for tissue engineering


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Numerous studies have shown that cell cultures in tissue culture plates and flasks do not reproduce the in vivo growth behavior of cells and have a poor correlation with animal as well human clinical data. Additive three-dimensional (3D) tissue-printing technology developed by regenHU Ltd, Villaz-St-Pierre, Switzerland, provides a promising new tool for tissue engineers, capable of creating organotypic tissues with true-to-life morphology that better mirrors the environment experienced by cells in vivo. Several studies show that tissues created using regenHU's BioFactory® and 3DDiscovery® bio-printing platforms can model natural cell behavior, intercellular interactions and differentiation processes.

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The well-defined structure of 3D-printed tissue enables the control and study of biological an mechanical cell/molecule interaction processes

The key advantage of this new tissue manufacturing technology resides in the fact that regenHU's bio-printer builds up tissue in a layer-upon-layer process. Thus, cells, signal molecules, and biomaterials, such as biopolymers, synthetic or natural hydrogels or calcium phosphates, form a highly dynamic network of proteins and signal transduction pathways. The printed, 3D cell-cell and cell-extracellular matrix closely resembles natural tissue. Its well-defined structure enables the control and study of biological and mechanical cell/molecule interaction processes.

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Matrix stiffness, cell adhesion and the ability to create pre-defined 3D shapes can be tailored with regenHU's BioInk.

For its 3D tissue-printing platform, regenHU has created BioInk®, a hydrogel that promotes cell growth for different cells by providing cell adhesion sites and mimicking the natural extracellular matrix. Bioink® is available as a ready-to-use solution to print 3D tissues. With BioInk®, critical parameters such as matrix stiffness, cell adhesion and the ability to create pre-defined 3D shapes with tissue-printing devices can be tailored. The BioInk® cartridges are specifically designed for BioFactory® and 3DDiscovery® tissue printers, enabling researchers to create composite tissue models with full spatial control of the allocation and distribution of bioactive components.

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Top view of a printed dermis equivalent. Fibroblasts printed in a layer-by-layer mode in combination with BioInk®. The dermis equivalent was cultivated for 7 days and the fibroblasts are stained with MTT (dark cells; live stain).

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HE-staining of a printed dermis equivalent cultivated for 5 days. Cross section of a dermis equivalent harboring fibroblasts. The layer-by-layer printing mode is still visible. Pictures kindly provided by ZHAW, Wädenswil, Institute of Chemistry and Biological Chemistry, Tissue Engineering group, Prof. Dr. Ursula Graf-Hausner.

For more information, you can contact:

E-mail: info@regenhu.com

http://www.regenhu.com

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