Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures (pages 500–515)
Dr. Juan A. Hernández Bort, Matthias Hackl, Helga Höflmayer, Vaibhav Jadhav, Eva Harreither, Niraj Kumar, Wolfgang Ernst, Johannes Grillari and Nicole Borth
Article first published online: 5 AUG 2011 | DOI: 10.1002/biot.201100143
The cellular transcriptome is dynamic, changing in response to external and internal stimuli and thus reflecting the current functional capacity of cells as well their ability to adapt to a changing environment. In this study, the authors use microarrays expression profiling to identify mRNA and miRNA that are differentially expressed during CHO-K1 suspension cell batch cultures. The results show the importance of time course experiments for comparison of phenotypes at different culture conditions.