Addressable self-immobilization of lactate dehydrogenase across multiple length scales (pages 262–272)
Sibel Cetinel, H. Burak Caliskan, Deniz T. Yucesoy, A. Senem Donatan, Esra Yuca, Mustafa Urgen, Nevin G. Karaguler and Prof. Candan Tamerler
Article first published online: 5 FEB 2013 | DOI: 10.1002/biot.201100502
Inorganic-binding peptide tags provide a singk-step approach for self-organization of enzymes on solid surfaces. In this article, gold-binding peptide (AuBP2) was genetically incorporated to L-lactate dehydrogenase (LDH). Enhanced enzymatic activity was observed on a variety of gold surfaces ranging from nanoparticles to electrodes. Redox catalysis efficiency obtained by the immobilized fusion enzyme suggests that the proposed peptide-mediated path could be extended to a variety of applications.