Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification (pages 709–717)
Luís Borlido, Leila Moura, Ana M. Azevedo, Ana C. A. Roque, Dr. Maria R. Aires-Barros and Dr. José Paulo S. Farinha
Article first published online: 11 APR 2013 | DOI: 10.1002/biot.201200329
Monoclonal antibodies are very important therapeutic proteins with wide applications in medicine. One of the challenges facing large-scale production of monoclonal antibodies is separation efficiency during the purification process. High binding capacities of small sized magnetic adsorbents can potentially circumvent foreseeable capacity limitations in the downstream processing of monoclonal antibodies. With stimuli-responsive magnetic particles the authors have obtained high binding capacities and fast separation times that can be improved by stimuli-induced particle aggregation.