Biotechnology Journal

Cover image for Biotechnology Journal

Special Issue: Biochemical Engineering Sciences

June 2013

Volume 8, Issue 6

Pages 633–749, A1–A8

  1. Cover Picture

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    3. Editorial Board
    4. Editorial
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    6. Contents
    7. BiotecVisions
    8. Forum
    9. Reviews
    10. Research Articles
    11. Technical Report
    12. Research Article
    13. Meetings
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      Biochemical Engineering Sciences

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201390027

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      This Special Issue is a collection of the latest research in biochemical engineering science presented at the 9th ESBES Conference in Istanbul, Turkey, in 2012. The cover illustrates the development in biochemical engineering science by showing symbols for several biochemical engineering subdisciplines, such as process engineering, strain and drug design, and material science, linked by covalent bonds in a hypothetical biological molecule.

  2. Editorial Board

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      Editorial Board: Biotechnology Journal 6/2013 (page 633)

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201390031

  3. Editorial

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      Editorial: ESBES – European Society of Biochemical Engineering Sciences (pages 634–635)

      Prof. Guilherme Ferreira and Prof. Alois Jungbauer

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201300220

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      The latest ESBES special issue on “Biochemical Engineering Sciences” is edited by Prof. Guilherme Ferreira (Chairman, ESBES) and Prof. Alois Jungbauer (co-Editor-in-Chief, Biotechnology Journal). This special issue comprises the latest research in biochemical engineering science presented at the 9th ESBES Conference held in Istanbul, Turkey in 2012.

  4. In this issue

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    3. Editorial Board
    4. Editorial
    5. In this issue
    6. Contents
    7. BiotecVisions
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    9. Reviews
    10. Research Articles
    11. Technical Report
    12. Research Article
    13. Meetings
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  5. Contents

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      Contents: Biotechnology Journal 6/2013 (pages 637–638)

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201390029

  6. BiotecVisions

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  7. Forum

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      Meeting report: 8th HIC/RPC Bioseparation Conference (pages 639–640)

      Regina Roemling and Alois Jungbauer

      Article first published online: 26 APR 2013 | DOI: 10.1002/biot.201300123

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      The 8th HIC/RPC Bioseparation Conference was held from March 3–7, 2013 at the Westin Savannah Harbor Resort in Savannah, Georgia, USA. Read this meeting report to find out more on the latest developments in chromatography for bioprocess applications.

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      Biotechnology essay competition: Biotechnology and sustainable food practices (pages 641–643)

      Judy Peng, Helena Schoeb and Gina Lee

      Article first published online: 6 MAY 2013 | DOI: 10.1002/biot.201300180

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      Biotechnology Journal announces our second biotechnology essay competition with the theme “biotechnology and sustainable food practices”, open to all undergraduate students.

  8. Reviews

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    1. Bioreactor design for clinical-grade expansion of stem cells (pages 644–654)

      Francisco F. dos Santos, Pedro Z. Andrade, Cláudia Lobato da Silva and Prof. Joaquim M.S. Cabral

      Article first published online: 26 APR 2013 | DOI: 10.1002/biot.201200373

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      With the current pace of progress, in a few years, stem cell-based therapy should be able to respond to the market demand for safe, robust and clinically efficient stem cell-based therapeutics. Bioreactors provide a viable alternative to the traditional static culture systems in that they provide the required scalability, monitoring and control tools, as well as the operational flexibility to be adapted to the different requirements of various clinical applications.

    2. Host cell protein analysis in therapeutic protein bioprocessing – methods and applications (pages 655–670)

      Anne Luise Tscheliessnig, Julita Konrath, Ron Bates and Prof. Alois Jungbauer

      Article first published online: 22 FEB 2013 | DOI: 10.1002/biot.201200018

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      Host cell protein content is one of the three most important quality criteria of biopharmaceuticals. In this review, the authors compare different methods for analysis of host cell proteins, and how cell lines, fermentation conditions, and unit operations influence host cell protein distribution during the bioprocess chain.

    3. Functional monolithic platforms: Chromatographic tools for antibody purification (pages 671–681)

      Telma Barroso, Abid Hussain, Ana C. A. Roque and Prof. Ana Aguiar-Ricardo

      Article first published online: 6 MAY 2013 | DOI: 10.1002/biot.201200328

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      Monoliths are an efficient platform for antibody purification. The use of monoclonal antibodies (MAbs) and engineered antibody structures as effective therapeutics for cancer, autoimmune, inflammation and infectious diseases has increased exponentially with an annual market of tens of billions of US dollars. Thus, several approaches involving monoliths for the production and purification of large antibody quantities with defined clinical and performance requirements have been developed. This review comprises a comprehensive analysis of proposed solutions highlighting the remarkable achievements of this platform for the antibody purification field even at a commercial level.

    4. Large-scale production of diesel-like biofuels – process design as an inherent part of microorganism development (pages 682–689)

      Dr. Maria C. Cuellar, Joseph J. Heijnen and Luuk A. M. van der Wielen

      Article first published online: 6 MAY 2013 | DOI: 10.1002/biot.201200319

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      In order to increase industrial implementation, process design should be imposed at the start of bioprocess development. This allows for timely generation of large scale-based targets for microorganism development and novel process configurations. In this review, the authors analyze the microbial production of diesel-like molecules, showing that microorganism development should be directed towards anaerobic production, increasing robustness at extreme process conditions and tailoring cell surface properties, while novel process configurations integrating fermentation and product recovery, cell reuse and low-cost technologies for product separation are mandatory.

  9. Research Articles

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    1. Acoustic detection of cell adhesion to a coated quartz crystal microbalance – implications for studying the biocompatibility of polymers (pages 690–698)

      Ana-Carina Da-Silva, Sandra S. Soares and Prof. Guilherme N. M. Ferreira

      Article first published online: 11 APR 2013 | DOI: 10.1002/biot.201200320

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      The propagation of acoustic waves from a quartz crystal microbalance (QCM) sensor can be used to monitor the growth of adherent cells. This study demonstrates that the signal generated by QCM sensors changes according to cell morphology, a unique property that can be explored for surface material biocompatibility studies. QCM sensors therefore have great potential in tissue engineering applications.

    2. Harnessing Candida tenuis and Pichia stipitis in whole-cell bioreductions of o-chloroacetophenone: Stereoselectivity, cell activity, in situ substrate supply and product removal (pages 699–708)

      Christoph Gruber, Stefan Krahulec, Bernd Nidetzky and Dr. Regina Kratzer

      Article first published online: 13 MAY 2013 | DOI: 10.1002/biot.201200322

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      Enantiopure alcohols are frequently produced by the bioreduction of prochiral ketones. The application of native strains as whole cell catalysts provides a simple method towards chiral alcohols consisting of cell cultivation, whole cell reduction and product analysis. (S)-1-(2-chlorophenyl)ethanol, the product of the presented bioreduction, is a key intermediate in the synthesis of a new class of chemotherapeutic substances (polo-like kinase 1 inhibitors).

    3. Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification (pages 709–717)

      Luís Borlido, Leila Moura, Ana M. Azevedo, Ana C. A. Roque, Dr. Maria R. Aires-Barros and Dr. José Paulo S. Farinha

      Article first published online: 11 APR 2013 | DOI: 10.1002/biot.201200329

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      Monoclonal antibodies are very important therapeutic proteins with wide applications in medicine. One of the challenges facing large-scale production of monoclonal antibodies is separation efficiency during the purification process. High binding capacities of small sized magnetic adsorbents can potentially circumvent foreseeable capacity limitations in the downstream processing of monoclonal antibodies. With stimuli-responsive magnetic particles the authors have obtained high binding capacities and fast separation times that can be improved by stimuli-induced particle aggregation.

    4. Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases (pages 719–729)

      Veronika Stepankova, Jiri Damborsky and Dr. Radka Chaloupkova

      Article first published online: 21 MAR 2013 | DOI: 10.1002/biot.201200378

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      Organic co-solvents can improve activity and enantioselectivity of haloalkane dehalogenases: in an effort to choose the most compatible organic co-solvent for haloalkane dehalogenases, the effects of fourteen co-solvents on the activity, stability and enantioselectivity of three model enzymes were systematically evaluated. The study demonstrates the favorable effect of the co-solvents at low concentrations on both activity and enantioselectivity of one of the tested haloalkane dehalogenases, DbjA, making this enzyme a potential catalyst for commercial biocatalytic applications.

  10. Technical Report

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    1. Rapid screening of potential autophagic inductor agents using mammalian cell lines (pages 730–737)

      Dr. Waleska K. Martins, Divinomar Severino, Cleidiane Souza, Beatriz S. Stolf and Maurício S. Baptista

      Article first published online: 21 MAR 2013 | DOI: 10.1002/biot.201200306

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      Although recent progress in understanding the molecular basis of autophagy has demonstrated its importance for human health, affordable screening techniques to identify autophagy in vitro are not yet available. In this article, the authors describe a novel in vitro strategy for rapid screening of potential autophagic inductor agents using mammalian cell lines. The proposed strategy is very useful for drug discovery, allowing the investigation of potential autophagic inductors in a single microplate. The conceptual framework of the method is the superior uptake of Neutral Red (NR) in cells engaging in autophagy.

  11. Research Article

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      Designing a fully automated multi-bioreactor plant for fast DoE optimization of pharmaceutical protein production (pages 738–747)

      Jens Fricke, Kristof Pohlmann, Nils A. Jonescheit, Andree Ellert, Burkhard Joksch and Prof. Reiner Luttmann

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201200190

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      In this report an approach for a fast and reproducible optimization of protein production in an in-house designed small-scale BIOSTAT® multi-bioreactor plant is described. Advanced DoE-software solutions enable the implementation of fully automated cultivation procedures. Using extended PAT-devices, a doubling of intact protein secretion productivity within seven 24 h cultivation cycles was observed. This approach opens up a promising cost benefit for pharmaceutical protein production stakeholders by reducing investigation time.

  12. Meetings

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      Meetings and Conferences: Biotechnology Journal 6/2013 (pages 748–749)

      Article first published online: 6 JUN 2013 | DOI: 10.1002/biot.201390030

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