Timo R. Maarleveld, Ruchir A. Khandelwal, Brett G. Olivier, Bas Teusink and Prof. Frank J. Bruggeman
Metabolism is responsible for the supply of energy and building blocks for cell growth and maintenance. One way to explore metabolism is by using computational modeling approaches. In this review, the authors use simple metabolic networks to explain the state-of-the-art computational modeling techniques. Basic concepts, such as stoichiometry, chemical moiety conservation, flux modes, flux balance analysis, and flux solution spaces are explained with simple, illustrative examples. This review will provide rigorous and quantitative hypotheses and fundamental understanding for metabolic networks studies.