FRET-based and other fluorescent proteinase probes (pages 266–281)
Hai-Yu Hu, Stefanie Gehrig, Gregor Reither, Devaraj Subramanian, Marcus A. Mall, Oliver Plettenburg and Carsten Schultz
Version of Record online: 27 JAN 2014 | DOI: 10.1002/biot.201300201
Fluorescent probes, in particular ratiometric FRET (Förster resonance energy transfer) probes, made it possible to observe enzyme activities in intact cells and living organisms. In this review, the authors summarize the various types of proteinase-sensitive fluorescent FRET probes as well as their applications. In the future, these probes will help determine disease states of patients and evaluate therapeutic effects of drugs and drug candidates.