Peptide microarrays enable rapid mimotope optimization for pharmacokinetic analysis of the novel therapeutic antibody IMAB362 (pages 545–554)
Karsten Schnatbaum, Hans-Ulrich Schmoldt, Matin Daneschdar, Laura M. Plum, Janina Jansong, Johannes Zerweck, Yvonne Kühne, Antonia Masch, Holger Wenschuh, Markus Fiedler, Özlem Türeci, Ugur Sahin and Ulf Reimer
Article first published online: 7 MAR 2014 | DOI: 10.1002/biot.201300456
Detection of therapeutic antibodies against membrane proteins for preclinical studies is challenging because the natural target is not readily available. In this study, mimotopes for the antibody IMAB362 (Claudiximab) were discovered and optimized using phage display and peptide microarrays and the best mimotope was successfully used for the peptide ELISA-based quantification of IMAB362. The described process efficiently provides mimotopes for targets which are difficult to produce or handle.