Changes in the refractive index ellipsoid isotropies, symmetric anisotropies, and depolarization ratios of potassium hyaluronate solutions as a function of pH
Article first published online: 1 FEB 2004
Copyright © 1979 John Wiley & Sons, Inc.
Volume 18, Issue 2, pages 351–358, February 1979
How to Cite
Barrett, T. W. (1979), Changes in the refractive index ellipsoid isotropies, symmetric anisotropies, and depolarization ratios of potassium hyaluronate solutions as a function of pH. Biopolymers, 18: 351–358. doi: 10.1002/bip.1979.360180211
- Issue published online: 1 FEB 2004
- Article first published online: 1 FEB 2004
- Manuscript Accepted: 5 JUN 1978
- Manuscript Revised: 24 MAY 1978
- Manuscript Received: 4 NOV 1977
A new method is presented for studying the tertiary and possibly the secondary structure of a biopolymer, which is applied to the pH-dependent structural transition of potassium hyaluronate solutions. The method involves a new application of Dirac dispersion theory polarization dependence measures to isotropic and anisotropic components of the refractive index ellipsoid. Changes in the dipole moment may also be observed by this method. The pH-induced changes in viscosity and optical properties of hyaluronic acid previously reported appear to be dependent on changes in secondary and tertiary structure.