Total synthesis by modern chemical ligation methods and high resolution (1.1 Å) X-ray structure of ribonuclease A

Authors

  • David J. Boerema,

    1. Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
    2. Institute for Biophysical Dynamics, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
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  • Valentina A. Tereshko,

    1. Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
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  • Stephen B. H. Kent

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
    2. Institute for Biophysical Dynamics, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
    3. Department of Chemistry, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
    • Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, 929 East 57th Street, Chicago, IL 60637
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  • This paper is dedicated to the memory of Bruce Merrifield (1921–2006), an extraordinary scientist and an exacting mentor.

Abstract

The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90:278–286, 2008.

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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