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Contemporary Problems in RNA Science—Special Focus
Applications of isothermal titration calorimetry in RNA biochemistry and biophysics
Article first published online: 1 AUG 2007
DOI: 10.1002/bip.20816
Copyright © 2007 Wiley Periodicals, Inc.
Issue
Biopolymers
Special Issue: Contemporary Problems in RNA Science—Special Focus
Volume 87, Issue 5-6, pages 293–301, 5 - 15 December 2007
Additional Information
How to Cite
Feig, A. L. (2007), Applications of isothermal titration calorimetry in RNA biochemistry and biophysics. Biopolymers, 87: 293–301. doi: 10.1002/bip.20816
Publication History
- Issue published online: 29 OCT 2007
- Article first published online: 1 AUG 2007
- Manuscript Revised: 19 JUL 2007
- Manuscript Accepted: 19 JUL 2007
- Manuscript Received: 29 JUN 2007
Funded by
- NIH. Grant Numbers: GM065430, GM075068
- Abstract
- Article
- References
- Cited By
Keywords:
- ITC;
- thermodynamics;
- binding;
- folding
Abstract
Isothermal titration calorimetry (ITC) has been applied to the study of proteins for many years. Its use in the biophysical analysis of RNAs has lagged significantly behind its use in protein biochemistry, however, in part because of the relatively large samples required. As the instrumentation has become more sensitive, the ability to obtain high quality data on RNA folding and RNA ligand interactions has improved dramatically. This review provides an overview of the ITC experiment and describes recent work on RNA systems that have taken advantage of its versatility for the study of small molecule binding, protein binding, and the analysis of RNA folding. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 293–301, 2007.
This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com