Dissecting membrane protein architecture: An annotation of structural complexity

Authors

  • Jaime Arce,

    1. LISM/CNRS Aix-Marseille Université, Laboratoire d'Ingénierie de Systèmes Macromoléculaires (UPR9027), Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille cedex 2013402, France
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  • James N. Sturgis,

    1. LISM/CNRS Aix-Marseille Université, Laboratoire d'Ingénierie de Systèmes Macromoléculaires (UPR9027), Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille cedex 2013402, France
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  • Jean-Pierre Duneau

    Corresponding author
    1. LISM/CNRS Aix-Marseille Université, Laboratoire d'Ingénierie de Systèmes Macromoléculaires (UPR9027), Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille cedex 2013402, France
    • LISM/CNRS Aix-Marseille Université, Laboratoire d'Ingénierie de Systèmes Macromoléculaires (UPR9027), Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille cedex 2013402, France
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Abstract

α-Helical membrane proteins exist in an anisotropic environment which strongly influences their folding, stability, and architecture, which is far more complex than a simple bundle of transmembrane helices, notably due to helix deformations, prosthetic groups and extramembrane structures. However, the role and the distribution of such heterogeneity in the supra molecular organization of membrane proteins remains poorly investigated. Using a nonredundant subset of α-helical membrane proteins, we have annotated and analyze the statistics of several types of new elements such as incomplete helices, intramembrane loops, helical extensions of helical transmembrane domains, extracellular loops, and helices lying parallel to the membrane surface. The relevance of the annotation scheme was studied using residue composition, statistics, physical chemistry, and symmetry of their distribution in relation to the immediate membrane environment. Calculation of hydrophobicity using different scales show that different structural elements appear to have affinities coherent with their position in the membrane. Examination of the annotation scheme suggests that there is considerable information content in the amino acid compositions of the different elements suggesting that it might be useful for structural prediction. More importantly, the proposed annotation will help to decipher the complex hierarchy of interactions involved in membrane protein architecture. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 815–829, 2009.

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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