Comparison of the internalization of targeted dendrimers and dendrimer-entrapped gold nanoparticles into cancer cells

Authors

  • Xiangyang Shi,

    Corresponding author
    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China
    2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China
    • State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China
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    • X. Shi and S. H. Wang equally contributed to this work

  • Su He Wang,

    Corresponding author
    1. Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, 109 Zina Pitcher Place, 4037 BSRB, Ann Arbor, MI 48109
    • Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109
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    • X. Shi and S. H. Wang equally contributed to this work

  • Inhan Lee,

    1. Michigan Center for Biological Information and Department of Psychiatry, University of Michigan, 3600 Green Court, Suite 700, Ann Arbor, MI 48105
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  • Mingwu Shen,

    1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China
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  • James R. Baker Jr.

    Corresponding author
    1. Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, 109 Zina Pitcher Place, 4037 BSRB, Ann Arbor, MI 48109
    • Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109
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Abstract

Dendrimer-based nanotechnology significantly advances the area of targeted cancer imaging and therapy. Herein, we compared the difference of surface acetylated fluorescein isocyanate (FI) and folic acid (FA) modified generation 5 (G5) poly(amidoamine) dendrimers (G5.NHAc-FI-FA), and dendrimer-entrapped gold nanoparticles with similar modifications ([(Au0)51.2-G5.NHAc-FI-FA]) in terms of their specific internalization to FA receptor (FAR)-overexpressing cancer cells. Confocal microscopic studies show that both G5.NHAc-FI-FA and [(Au0)51.2-G5.NHAc-FI-FA] exhibit similar internalization kinetics regardless of the existence of Au nanoparticles (NPs). Molecular dynamics simulation of the two different nanostructures reveals that the surface area and the FA moiety distribution from the center of the geometry are slightly different. This slight difference may not be recognized by the FARs on the cell membrane, consequently leading to similar internalization kinetics. This study underlines the fact that metal or inorganic NPs entrapped within dendrimers interact with cells in a similar way to that of dendrimers lacking host NPs. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 936–942, 2009.

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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