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Physical and chemical aspects of stabilization of compounds in silk †
Article first published online: 23 JAN 2012
Copyright © 2012 Wiley Periodicals, Inc.
Special Issue: Silks: Properties and Uses of Natural and Designed Variants
Volume 97, Issue 6, pages 479–498, June 2012
How to Cite
Pritchard, E. M., Dennis, P. B., Omenetto, F., Naik, R. R. and Kaplan, D. L. (2012), Physical and chemical aspects of stabilization of compounds in silk . Biopolymers, 97: 479–498. doi: 10.1002/bip.22026
- Issue published online: 20 MAR 2012
- Article first published online: 23 JAN 2012
- Manuscript Accepted: 14 DEC 2011
- Manuscript Received: 13 DEC 2011
- silk fibroin;
- drug delivery;
- tissue engineering
The challenge of stabilization of small molecules and proteins has received considerable interest. The biological activity of small molecules can be lost as a consequence of chemical modifications, while protein activity may be lost due to chemical or structural degradation, such as a change in macromolecular conformation or aggregation. In these cases, stabilization is required to preserve therapeutic and bioactivity efficacy and safety. In addition to use in therapeutic applications, strategies to stabilize small molecules and proteins also have applications in industrial processes, diagnostics, and consumer products like food and cosmetics. Traditionally, therapeutic drug formulation efforts have focused on maintaining stability during product preparation and storage. However, with growing interest in the fields of encapsulation, tissue engineering, and controlled release drug delivery systems, new stabilization challenges are being addressed; the compounds or protein of interest must be stabilized during: (1) fabrication of the protein or small molecule-loaded carrier, (2) device storage, and (3) for the duration of intended release needs in vitro or in vivo. We review common mechanisms of compound degradation for small molecules and proteins during biomaterial preparation (including tissue engineering scaffolds and drug delivery systems), storage, and in vivo implantation. We also review the physical and chemical aspects of polymer-based stabilization approaches, with a particular focus on the stabilizing properties of silk fibroin biomaterials. © 2011 Wiley Periodicals, Inc. Biopolymers 97: 479–498, 2012.