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Influence of poly(n-isopropylacrylamide)–CNT–polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability

Authors

  • Ashutosh Tiwari,

    Corresponding author
    1. Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, S-58183 Linköping, Sweden
    2. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
    • Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, S-58183 Linköping, Sweden
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  • Yashpal Sharma,

    1. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
    2. Department of Chemistry, G. J. University of Science and Technology, Hisar 125-001, India
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  • Shinya Hattori,

    1. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
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  • Dohiko Terada,

    1. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
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  • Ashok K. Sharma,

    1. Department of Materials Science and Nanotechnology, D. C. R. University of Science and Technology, Murthal 131-039, India
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  • Anthony P. F. Turner,

    1. Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, S-58183 Linköping, Sweden
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  • Hisatoshi Kobayashi

    Corresponding author
    1. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
    2. JST-CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan
    • International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan
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  • This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

Abstract

This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)–CNT–polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)–CNT–polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400–500 μm; L: 6–20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)–CNT–polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)–CNT–polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)–CNT–polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)–CNT–polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)–CNT–polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 334–341, 2013.

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