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Keywords:

  • free-energy landscape;
  • conformational states;
  • ribosome;
  • single-molecule FRET

ABSTRACT

This article addresses recent developments in cryo-electron microscopy and single-particle reconstruction of macromolecules. With the advent of powerful classification techniques, it is now possible to extract and visualize multiple conformers contained within the same dataset. It is discussed how and to what extent this technique can be used in the study of the dynamics of molecular machines. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 832–836, 2013.