A personal perspective on chemistry-driven RNA research

Authors

  • Ronald Micura,

    Corresponding author
    • Institute of Organic Chemistry, Center for Molecular Biosciences (CMBI), Center for Chemistry and Biomedicine (CCB), University of Innsbruck, Innsbruck, Austria
    Search for more papers by this author
  • Christoph Kreutz,

    Corresponding author
    • Institute of Organic Chemistry, Center for Molecular Biosciences (CMBI), Center for Chemistry and Biomedicine (CCB), University of Innsbruck, Innsbruck, Austria
    Search for more papers by this author
  • Kathrin Breuker

    Corresponding author
    • Institute of Organic Chemistry, Center for Molecular Biosciences (CMBI), Center for Chemistry and Biomedicine (CCB), University of Innsbruck, Innsbruck, Austria
    Search for more papers by this author

  • This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

Correspondence to: R. Micura; e-mail: ronald.micura@uibk.ac.at (or) C. Kreutz; e-mail: christoph.kreutz@uibk.ac.at (or) K. Breuker; e-mail: kathrin.breuker@uibk.ac.at

ABSTRACT

In this mini review, we discuss how our understanding of ribonucleic acid (RNA) properties becomes significantly deepened when a broad range of modern chemical and biophysical methods is applied. We span our perspective from RNA solid-phase synthesis and site-specific labeling to single-molecule fluorescence-resonance-energy-transfer imaging and NMR spectroscopy approaches to explore the dynamics of RNA over a broad timescale. We then move on to Fourier-transform-ion-cyclotron-resonance mass spectrometry (FT-ICR-MS) as a powerful technique for RNA sequencing and modification analysis. The novel methodological developments are discussed for selected biological systems that include the thiamine-pyrophosphate riboswitch, HIV and ribosomal A-site RNA, and transfer RNA. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 1114–1123, 2013.

Ancillary