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Reentrant condensation of lysozyme: Implications for studying dynamics of lysozyme in aqueous solutions of lithium chloride
Article first published online: 25 MAR 2014
Copyright © 2013 Wiley Periodicals Inc.
Volume 101, Issue 6, pages 624–629, June 2014
How to Cite
Mamontov, E. and O'Neill, H. (2014), Reentrant condensation of lysozyme: Implications for studying dynamics of lysozyme in aqueous solutions of lithium chloride. Biopolymers, 101: 624–629. doi: 10.1002/bip.22430
- Issue published online: 25 MAR 2014
- Article first published online: 25 MAR 2014
- Accepted manuscript online: 30 OCT 2013 04:43AM EST
- Manuscript Accepted: 11 OCT 2013
- Manuscript Received: 1 AUG 2013
- ORNL's Center for Structural Molecular Biology, Office of Biological and Environmental Research, US DOE. Grant Number: ERKP291
- ORNL, UTBattelle, LLC, US DOE. Grant Number: DE-AC05-00OR22725
- neutron scattering
Recent studies have outlined the use of eutectic solutions of lithium chloride in water to study microscopic dynamics of lysozyme in an aqueous solvent that is remarkably similar to pure water in many respects, yet allows experiments over a wide temperature range without solvent crystallization. The eutectic point in a (H2O)R(LiCl) system corresponds to R ≈ 7.3, and it is of interest to investigate whether less-concentrated aqueous solutions of LiCl could be used in low-temperature studies of a solvated protein. We have investigated a range of concentrations of lysozyme and LiCl in aqueous solutions to identify systems that do not show phase separation and avoid solvent crystallization on cooling down. Compared to the lysozyme concentration in solution, the concentration of LiCl in the aqueous solvent plays the major role in determining systems suitable for low-temperature studies. We have observed interesting and rich phase behavior reminiscent of reentrant condensation of proteins. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 624–629, 2014.