Absorption spectra of nucleotides, polynucleotides, and nucleic acids in the far ultraviolet
Article first published online: 1 FEB 2004
Copyright © 1963 John Wiley & Sons, Inc.
Volume 1, Issue 3, pages 193–208, June 1963
How to Cite
Voet, D., Gratzer, W. B., Cox, R. A. and Doty, P. (1963), Absorption spectra of nucleotides, polynucleotides, and nucleic acids in the far ultraviolet. Biopolymers, 1: 193–208. doi: 10.1002/bip.360010302
- Issue published online: 1 FEB 2004
- Article first published online: 1 FEB 2004
- Manuscript Received: 4 JAN 1963
Ultaviolet absorption spectra down to 185 mμ are presented for a number of nucleic acids and synthetic polynucleotides in the helical and random forms, and of their constituent mononucleotides, as well as the corresponding nucleosides and free bases. A short wavelength absorption band is in all cases observed with a maximum varying between about 187 and 201 mμ. These bands are more intense than those near 260 mμ. The helical polymers show a hypochromic effect in this region of a somewhat similar magnitude to that in the 260 mμ region. Other spectral phenomena accompanying denaturation and degradation are described.