Part XXIII in the series, “Raman Spectral Studies of Nucleic Acids.” For part XXII in this series, see Thomas, G. J., Jr. & Ferreira, S. A. (1982) J. Raman Spectrosc.12, 122–124.
Characterization of DNA structures by laser Raman spectroscopy†
Article first published online: 1 FEB 2004
Copyright © 1984 John Wiley & Sons, Inc.
Volume 23, Issue 2, pages 235–256, February 1984
How to Cite
Prescott, B., Steinmetz, W. and Thomas, G. J. (1984), Characterization of DNA structures by laser Raman spectroscopy. Biopolymers, 23: 235–256. doi: 10.1002/bip.360230206
- Issue published online: 1 FEB 2004
- Article first published online: 1 FEB 2004
- Manuscript Accepted: 23 MAY 1983
- Manuscript Received: 17 DEC 1982
Oriented fibers drawn from aqueous gels of calf-thymus DNA were maintained at constant relative humidites of 75 and 92% to yield canonical A-DNA and B-DNA structures, respectively. Raman spectra of the two forms of DNA were recorded over the spectral range 300–4000 cm−1. The authenticated DNA fibers were deuterated in hygrostatic cells containing D2O at appropriate relative humidities, and the corresponding spectra of deuterated DNAs were also obtained. The spectra reveal all of the Raman scattering frequencies and intensities characteristic of A- and B-DNA structures in both nondeuterated and deuterated froms, as well as the frequencies and intensities of adsorbed solvent molecules from which the hydration content of DNA fibers can be calculated. Numerous conformation-sensitive vibrational modes of DNA bases and phosphate groups have been identified throughout the 300–1700-cm−1 interval. Evidence has also been obtained for conformation sensitivity of deoxyribosyl CH stretching modes in the 2800–3000-cm−1 region. Raman lines of both the backbone and the bases are proposed as convenient indicators of A- and B-DNA structures. The results are extended to Z-DNA models investigated previously. Some implications of these findings for the determination of DNA or RNA structure from Raman spectra of nucleoproteins and viruses are considered.