Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. III. Estimation of the protein secondary structure
Version of Record online: 1 FEB 2004
Copyright © 1990 John Wiley & Sons, Inc.
Volume 30, Issue 13-14, pages 1273–1280, 1990
How to Cite
Kalnin, N. N., Baikalov, I. A. and Venyaminov, S. Yu. (1990), Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. III. Estimation of the protein secondary structure. Biopolymers, 30: 1273–1280. doi: 10.1002/bip.360301311
- Issue online: 1 FEB 2004
- Version of Record online: 1 FEB 2004
- Manuscript Accepted: 6 JUN 1990
- Manuscript Received: 21 JUN 1989
Infrared spectra of 13 globular proteins have been obtained in the 1800–1480-cm−1 region for H2O solutions. A method for estimating protein secondary structure from the ir spectrum has been developed. The method can also be used for estimating polypeptide and fibrous protein conformation. For the globular and fibrous proteins and polypeptides analyzed, the correlation coefficients between the ir and x-ray estimates of ordered helix, disordered helix, ordered β-structure, disordered β-structure, turns, and remainder were 0.98, 0.80, 0.99, 0.87, 0.90, and 0.92 respectively.