CD of proline-rich polypeptides: Application to the study of the repetitive domain of maize glutelin-2

Authors

  • F. Rabanal,

    1. Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Martí i Franqués, 1, 08028, Spain
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  • M. D. Ludevid,

    1. Centre de Investigació i Desenvolupament, CSIC, Jordi Girona, 18, 08034 Barcelona, Spain
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  • M. Pons,

    1. Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Martí i Franqués, 1, 08028, Spain
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  • E. Giralt

    Corresponding author
    1. Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Martí i Franqués, 1, 08028, Spain
    • Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Martí i Franqués, 1, 08028, Spain
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Abstract

An overview of CD of proline-rich peptides is reported. First, structural characteristics, theoretical CD studies, and the biological relevance of polyproline II structure in such peptides are discussed. Second, a CD study of peptides belonging to the repetitive domain of maize glutelin-2, H-(Val-His-Leu-Pro-Pro-Pro)n-OH (n = 3, 5, 8), is described. This series of peptides displayed the CD features of polyproline II structure in water (5°C, pH5). Moreover, it was shown that the addition of increasing amounts of the polyanionic molecule heparin forced a displacement of the conformational equilibrium of those peptides toward higher proportions of the polyproline II structure. In contrast, when the temperature is raised such a structure gradually disappears, leading to more disordered conformations. © 1993 John Wiley & Sons, Inc.

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