Quantification of toluene dioxygenase induction and kinetic modeling of TCE cometabolism by Pseudomonas putida TVA8

  1. J.T. Shingleton1,†,
  2. B.A. Applegate1,‡,
  3. A.J. Baker3,
  4. G.S. Sayler1,*,
  5. P.R. Bienkowski2

Article first published online: 13 NOV 2001

DOI: 10.1002/bit.10083

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 76, Issue 4, pages 341–350, December 2001

Additional Information

How to Cite

Shingleton, J.T., Applegate, B.A., Baker, A.J., Sayler, G.S. and Bienkowski, P.R. (2001), Quantification of toluene dioxygenase induction and kinetic modeling of TCE cometabolism by Pseudomonas putida TVA8. Biotechnol. Bioeng., 76: 341–350. doi: 10.1002/bit.10083

Author Information

  1. 1

    Center for Environmental Biotechnology, The University of Tennessee, 676 Dabney Hall, Knoxville, Tennessee 37996-1605; telephone: (865) 974-8080; fax: 865 974-8086

  2. 2

    Department of Chemical Engineering, The University of Tennessee, Knoxville, Tennessee 37996

  3. 3

    Mechanical and Aerospace and Engineering Science, The University of Tennessee, Knoxville, Tennessee 37996

  1. †

    Present address: Merck and Co., Inc., 3517 Radium Springs Road, Albany, GA 31705

  2. ‡

    Present address: Food Science Department, 1160 Food Science Bldg., Purdue University, West Lafayette, IN 47907–1160

*Center for Environmental Biotechnology, The University of Tennessee, 676 Dabney Hall, Knoxville, Tennessee 37996-1605; telephone: (865) 974-8080; fax: 865 974-8086

Publication History

  1. Issue published online: 13 NOV 2001
  2. Article first published online: 13 NOV 2001
  3. Manuscript Accepted: 26 JUL 2001
  4. Manuscript Received: 10 OCT 2000

Funded by

  • Waste Management Research and Education Institute, The University of Tennesseee

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Keywords:

  • biodegradation bioluminescence;
  • Pseudomonas putida;
  • tod-lux bioreporter;
  • toluene;
  • toluene dioxygenase activity;
  • trichloroethylene (TCE)

Abstract

As measured by the toluene-induced bioluminescent response of Pseudomonas putida TVA8 in batch experiments, toluene dioxygenase (Tod) enzyme activities are dependent on toluene concentration between 0 and 30 mg/L. To provide a measure of the Tod activity for use in Michaelis-Menten competitive-inhibition kinetics, a correlation between toluene concentration and induced Tod activity as measured by an induced bioluminescent response of P. putida TVA8 is presented as a nondimensional Tod activity parameter. A packed-bed, radial-flow bioreactor (RFB) using the bioreporter P. putida TVA8A serves as the model system for studying the effect of the enzyme activity parameter on model predictions of vapor-phase toluene oxidation and trichloroethylene (TCE) cometabolism. Mass balances were performed on a differential section of the RFB to describe the radial transport of vapor-phase toluene and TCE through a bulk gas phase and the concomitant biological reaction in a stationary biofilm phase. The finite-element Galerkin weak-statement formulation with first-order basis functions was used to find the optimum solution to the highly nonlinear, coupled equations. For this RFB system with toluene concentrations less than 1 mg/L in the bulk gas phase, the Tod activity parameter enables accurate predictions of steady-state TCE degradation rate (0.27 μg TCE/min). © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 341–350, 2001.

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