Enzymatic coupling of phenol vapors onto chitosan

  1. Li-Qun Wu1,
  2. Tianhong Chen1,2,
  3. Kimberlee K. Wallace2,
  4. Rafael Vazquez-Duhalt3,
  5. Gregory F. Payne1,2,*

Article first published online: 12 NOV 2001

DOI: 10.1002/bit.10099

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 76, Issue 4, pages 325–332, December 2001

Additional Information

How to Cite

Wu, L.-Q., Chen, T., Wallace, K. K., Vazquez-Duhalt, R. and Payne, G. F. (2001), Enzymatic coupling of phenol vapors onto chitosan. Biotechnol. Bioeng., 76: 325–332. doi: 10.1002/bit.10099

Author Information

  1. 1

    Center for Agricultural Biotechnology, 5115 Plant Sciences Building, University of Maryland Biotechnology Institute, College Park, Maryland telephone: 301-405-8389; fax: 301-314-9075

  2. 2

    Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250

  3. 3

    Instituto de Biotecnologia UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62250 Mexico

*Center for Agricultural Biotechnology, 5115 Plant Sciences Building, University of Maryland Biotechnology Institute, College Park, Maryland telephone: 301-405-8389; fax: 301-314-9075

Publication History

  1. Issue published online: 12 NOV 2001
  2. Article first published online: 12 NOV 2001
  3. Manuscript Accepted: 13 AUG 2001
  4. Manuscript Received: 4 MAY 2001

Funded by

  • Venture Innovations, National Science Foundation. Grant Number: NSF DMI-9901868

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Keywords:

  • chitosan;
  • gas phase;
  • phenols;
  • tyrosinase;
  • vapor phase

Abstract

Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed) onto chitosan films. First, phenol-trapping studies indicated that p-cresol can be removed from vapors if the vapors are contacted with tyrosinase-coated chitosan films. Second, the ultraviolet absorbance of tyrosinase-coated chitosan films changes dramatically when they are contacted with cresol-containing vapors, whereas control films are unaffected by contacting with cresol vapors. Third, pressure measurements indicate that tyrosinase-coated chitosan films only react with cresol vapors if the oxygen cosubstrate is present. Additional studies demonstrate the potential of tyrosinase-coated chitosan films/membranes for the detection and removal of phenol vapors. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 325–332, 2001.

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