Communication to the Editor

Bioengineering and characterization of DNA–protein assemblies floating on supported membranes

  1. Wilfrid Boireau1,
  2. Sophie Bombard2,
  3. Marie-Agnès Sari2,
  4. Denis Pompon1,*

Article first published online: 5 DEC 2001

DOI: 10.1002/bit.10111

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 77, Issue 2, pages 225–231, 20 January 2002

Additional Information

How to Cite

Boireau, W., Bombard, S., Sari, M.-A. and Pompon, D. (2002), Bioengineering and characterization of DNA–protein assemblies floating on supported membranes. Biotechnology and Bioengineering, 77: 225–231. doi: 10.1002/bit.10111

Author Information

  1. 1

    Laboratoire d'lngénierie des Protéines Membranaires, Centre de Génétique Moléculaire, CNRS, F-91198 Gif-sur-Yvette Cedex, France; téléphone: +33 169823680; fax: +33 169823160

  2. 2

    Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, UMR 8601, F-75006 Paris, France

*Laboratoire d'lngénierie des Protéines Membranaires, Centre de Génétique Moléculaire, CNRS, F-91198 Gif-sur-Yvette Cedex, France; téléphone: +33 169823680; fax: +33 169823160

Publication History

  1. Issue published online: 5 DEC 2001
  2. Article first published online: 5 DEC 2001
  3. Manuscript Accepted: 27 AUG 2001
  4. Manuscript Received: 24 APR 2001

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Keywords:

  • biosensor;
  • hybrid bilayer;
  • lateral mobility;
  • redox protein;
  • DNA chips;
  • nanotechnology;
  • plasmon resonance

Abstract

A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 225–231, 2002.

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