Engineering of baker's yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis Lipase A
Article first published online: 15 MAR 2002
Copyright © 2002 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 78, Issue 3, pages 339–345, 5 May 2002
How to Cite
Sánchez, M., Prim, N., Rández–Gil, F., Pastor, F. I. J. and Diaz, P. (2002), Engineering of baker's yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis Lipase A. Biotechnol. Bioeng., 78: 339–345. doi: 10.1002/bit.10201
- Issue published online: 15 MAR 2002
- Article first published online: 15 MAR 2002
- Manuscript Accepted: 13 NOV 2001
- Manuscript Received: 26 JUL 2001
- triacylglycerol hydrolase;
- heterologous expression;
- baker's yeast;
Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEplac112 and pUB110-derived vectors, respectively. Lipase activity analysis of the recombinant strains showed that the gene can be properly expressed in all hosts assayed, this being the first time a lipase from bacterial origin can be expressed in baker's S. cerevisiae strains. An important increase of lipase production was obtained in heterologous hosts with respect to that of parental strains, indicating that the described systems can represent a useful tool to enhance productivity of the enzyme for biotechnological applications, including the use of the lipase in bread making, or as a technological additive. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 339–345, 2002.