This article is a US Government Work and, as such, is in the public domain in the United States of America.
Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris†
Article first published online: 5 MAR 2003
Copyright © 2003 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 82, Issue 4, pages 438–444, 20 May 2003
How to Cite
Trinh, L. B., Phue, J. N. and Shiloach, J. (2003), Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol. Bioeng., 82: 438–444. doi: 10.1002/bit.10587
- Issue published online: 5 MAR 2003
- Article first published online: 5 MAR 2003
- Manuscript Accepted: 14 OCT 2002
- Manuscript Received: 21 APR 2002
- Pichia pastoris;
- growth strategies;
Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in the second phase, the yeast utilizes methanol which is used both as an inducer for the expression of the recombinant protein and as a carbon source. Since the yeast is sensitive to methanol concentration, the methanol is supplied gradually to the growing culture. Three methanol addition strategies were evaluated for the purpose of optimizing recombinant endostatin production. Two strategies were based on the yeast metabolism; one responding to the methanol consumption using a methanol sensor, and the other responding to the oxygen consumption. In these two strategies, the methanol supply is unlimited. The third strategy was based on a predetermined exponential feeding rate, controling the growth rate at 0.02 h−1, in this strategy the methanol supply is limited. Throughout the induction phase glycerol, in addition to methanol, was continuously added at a rate of 1 g L h−1. Total endostatin production was similar in all three strategies, (400 mg was obtained from 3 L initial volume), but the amount of methanol added and the biomass produced were lower in the predetermined rate method. This caused the specific production of endostatin per biomass and per methanol to be 2 times higher in the predetermined rate than in the other two methods, making the growth control strategy not only more efficient but also more convenient for downstream processing. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 438–444, 2003.