The use of dinoflagellate bioluminescence to characterize cell stimulation in bioreactors
Article first published online: 22 APR 2003
Copyright © 2003 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 83, Issue 1, pages 93–103, 5 July 2003
How to Cite
Chen, A. K., Latz, M. I. and Frangos, J. A. (2003), The use of dinoflagellate bioluminescence to characterize cell stimulation in bioreactors. Biotechnol. Bioeng., 83: 93–103. doi: 10.1002/bit.10647
- Issue published online: 22 APR 2003
- Article first published online: 22 APR 2003
- Manuscript Accepted: 11 DEC 2002
- Manuscript Received: 11 JUL 2002
- National Science Foundation. Grant Number: BES-9730782
- temporal gradients
Bioluminescent dinoflagellates are flow-sensitive marine organisms that produce light emission almost instantaneously upon stimulation by fluid shear in a shear stress dose-dependent manner. In the present study we tested the hypothesis that monitoring bioluminescence by suspended dinoflagellates can be used as a tool to characterize cellular response to hydrodynamic forces in agitated bioreactors. Specific studies were performed to determine: (1) impeller configurations with minimum cell activation, (2) correlations of cellular response and an integrated shear factor, and (3) the effect of rapid acceleration in agitation. Results indicated that (1) at a volumetric mass transfer coefficient of 3 × 10−4 s−1, marine impeller configurations were less stimulatory than Rushton configurations, (2) bioluminescence response and a modified volumetric integrated shear factor had an excellent correlation, and (3) rapid acceleration in agitation was highly stimulatory, suggesting a profound effect of temporal gradients in shear in increasing cell stimulation. By using bioluminescence stimulation as an indicator of agitation-induced cell stimulation and/or damage in microcarrier cultures, the present study allows for the verification of hypotheses and development of novel mechanisms of cell damage in bioreactors. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 93–103, 2003.