Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)
Article first published online: 19 AUG 2004
Copyright © 2004 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 87, Issue 6, pages 754–762, 20 September 2004
How to Cite
Schoevaart, R., Wolbers, M.W., Golubovic, M., Ottens, M., Kieboom, A.P.G., van Rantwijk, F., van der Wielen, L.A.M. and Sheldon, R.A. (2004), Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol. Bioeng., 87: 754–762. doi: 10.1002/bit.20184
- Issue published online: 19 AUG 2004
- Article first published online: 19 AUG 2004
- Manuscript Accepted: 11 MAY 2004
- Manuscript Received: 17 DEC 2003
The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated. © 2004 Wiley Periodicals, Inc.