Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase

Authors

  • Dong Hyun Kim,

    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
    2. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742,Korea
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  • Jin Hee Kim,

    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
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  • Seung Hwa Baek,

    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
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  • Jin Ho Seo,

    1. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742,Korea
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  • Yung Hee Kho,

    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
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  • Tae Kwang Oh,

    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
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  • Choong Hwan Lee

    Corresponding author
    1. Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
    • Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Korea; telephone: + 82 42 860-4294; fax: + 82 42 860-4595
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Abstract

Inhibitors of melanin biosynthesis were screened by using three different methods. The extract of Veratrum patulum contains hydroxystilbene compounds that are potent tyrosinase inhibitors. We evaluated the enzyme inhibitory property on the mushroom tyrosinase of hydroxystilbene compounds including resveratrol, oxyresveratrol, and their analogs. Biotransformation using cellulase of the whole extract brought about an increase in the inhibitory activity of the products on mushroom tyrosinase. The enhancement of tyrosinase inhibition is supposed to increase the concentration of aglycon, which has superior inhibitory activity to its glycoside. Eventually, melanin biosynthesis was inhibited by the enhanced tyrosinase inhibitory activity of the extract. This result indicated that deglycosylation of stilbene compounds has exerted more effective inhibition on the enzyme than that of the unprocessed plant extract. © 2004 Wiley Periodicals, Inc.

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