Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells
Article first published online: 14 OCT 2004
Copyright © 2004 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Special Issue: Stem Cell Bioengineering
Volume 88, Issue 3, pages 313–320, 5 November 2004
How to Cite
Gerecht-Nir, S., Cohen, S., Ziskind, A. and Itskovitz-Eldor, J. (2004), Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells. Biotechnol. Bioeng., 88: 313–320. doi: 10.1002/bit.20248
- Issue published online: 14 OCT 2004
- Article first published online: 14 OCT 2004
- Manuscript Accepted: 18 MAR 2004
- Manuscript Received: 19 DEC 2003
- National Institutes of Health; Technion Research and Development Foundation, Ltd.. Grant Number: 1RO1Hl73798-01
- alginate scaffold;
- human embryonic stem cells;
Differentiation of human embryonic stem cells (hESCs) can be instigated through the formation of embryo-like aggregates in suspension, termed human embryoid bodies (hEBs). Controlling cell aggregation and agglomeration during hEBs formation has a profound effect on the extent of cell proliferation and differentiation. In a previous work, we showed that control over hEBs formation and differentiation can be achieved via cultivation of hESC suspensions in a rotating bioreactor system. We now report that hEBs can be generated directly from hESC suspensions within three-dimensional (3D) porous alginate scaffolds. The confining environments of the alginate scaffold pores enabled efficient formation of hEBs with a relatively high degree of cell proliferation and differentiation; encouraged round, small-sized hEBs; and induced vasculogenesis in the forming hEBs to a greater extent than in static or rotating cultures. We therefore conclude that differentiation of hEBs can be induced and directed by physical constraints in addition to chemical cues. © 2004 Wiley Periodicals, Inc.