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The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production

Authors

  • Mohamed B Al-Fageeh,

    1. Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; telephone: +44 1227 823746; fax: +44 1227 763912
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  • Rosalyn J Marchant,

    1. Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; telephone: +44 1227 823746; fax: +44 1227 763912
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  • Martin J Carden,

    1. Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; telephone: +44 1227 823746; fax: +44 1227 763912
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  • C Mark Smales

    Corresponding author
    1. Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; telephone: +44 1227 823746; fax: +44 1227 763912
    • Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; telephone: +44 1227 823746; fax: +44 1227 763912.
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Abstract

There are a growing number of reports on the sub-physiological temperature culturing (<37°C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that exposing mammalian cells to sub-physiological temperatures invokes a coordinated cellular response involving modulation of the cell cycle, metabolism, transcription, translation, and the cell cytoskeleton. Opportunities currently exist for further enhancement of the cold-shock effect on recombinant protein production in mammalian cells through advancements in our understanding of the mechanisms involved in the cold-shock response. © 2005 Wiley Periodicals, Inc.

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